pEP4 E02S ET2K

LOCUS       pEP4 E02S ET2K               17522 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4 .
ACCESSION   .
VERSION     .
KEYWORDS    pEP4 E02S ET2K
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17522)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 17522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17522
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..1175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          244..1166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    2385..2958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2408..2960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    3977..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4641..5803
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4872..5794
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5946..8072
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     misc_feature    8163..8736
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8186..8738
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    10223..10357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      11088..12877
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(13179..15104)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(15543..15561)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15629..15733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             15734..16594
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15952..15971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16765..17353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17254..17273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
       61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt gggcccgcgg
      601 ccggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
      841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
      901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
      961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1081 cgttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
     1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
     1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
     1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
     1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
     1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
     1501 gacctctcag cctgagggtg aagcangagt cggggtggag agcaactccg atggggcctc
     1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
     1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
     1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
     1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
     1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
     1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
     1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
     1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
     2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
     2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
     2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
     2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
     2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
     2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
     2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
     3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
     3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
     3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
     3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
     3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
     3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
     3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
     3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
     3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
     3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
     3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
     3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
     3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
     3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
     3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
     3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4321 gcccgtcagg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgaccaat
     4381 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
     4441 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
     4501 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     4561 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
     4621 attattgact agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     4681 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     4741 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     4801 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     4861 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     4921 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
     4981 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
     5041 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5101 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5161 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
     5221 gcccgcggcc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5281 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5341 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5401 accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg
     5461 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5521 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5581 cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5641 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5701 gtaattctcg ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5761 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacacgtg gtcgcggcca
     5821 attctgcagt cgagctcaag cttcgaacta gggccgcgac tctagcgcta ccggactcag
     5881 atctcgagct caagcttcga attcgattga tccgatcccg cgaccggtcg accagctttg
     5941 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     6001 ttgaaaggag tgcctggggg aatattcctc tgatgagaaa ggcatattta aaaaaatgca
     6061 aggagtttca tcctgataaa ggaggagatg aagaaaaaat gaagaaaatg aatactctgt
     6121 acaagaaaat ggaagatgga gtaaaatatg ctcatcaacc tgactttgga ggcttctggg
     6181 atgcaactga gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     6241 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     6301 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     6361 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     6421 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     6481 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     6541 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     6601 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     6661 atccattttc tgttattgag gaaagtttgc caggtgggtt aaaggagcat gattttaatc
     6721 cagaagaagc agaggaaact aaacaagtgt cctggaagct tgtaacagag tatgcaatgg
     6781 aaacaaaatg tgatgatgtg ttgttattgc ttgggatgta cttggaattt cagtacagtt
     6841 ttgaaatgtg tttaaaatgt attaaaaaag aacagcccag ccactataag taccatgaaa
     6901 agcattatgc aaatgctgct atatttgctg acagcaaaaa ccaaaaaacc atatgccaac
     6961 aggctgttga tactgtttta gctaaaaagc gggttgatag cctacaatta actagagaac
     7021 aaatgttaac aaacagattt aatgatcttt tggataggat ggatataatg tttggttcta
     7081 caggctctgc tgacatagaa gaatggatgg ctggagttgc ttggctacac tgtttgttgc
     7141 ccaaaatgga ttcagtggtg tatgactttt taaaatgcat ggtgtacaac attcctaaaa
     7201 aaagatactg gctgtttaaa ggaccaattg atagtggtaa aactacatta gcagctgctt
     7261 tgcttgaatt atgtgggggg aaagctttaa atgttaattt gcccttggac aggctgaact
     7321 ttgagctagg agtagctatt gaccagtttt tagtagtttt tgaggatgta aagggcactg
     7381 gaggggagtc cagagatttg ccttcaggtc agggaattaa taacctggac aatttaaggg
     7441 attatttgga tggcagtgtt aaggtaaact tagaaaagaa acacctaaat aaaagaactc
     7501 aaatatttcc ccctggaata gtcaccatga atgagtacag tgtgcctaaa acactgcagg
     7561 ccagatttgt aaaacaaata gattttaggc ccaaagatta tttaaagcat tgcctggaac
     7621 gcagtgagtt tttgttagaa aagagaataa ttcaaagtgg cattgctttg cttcttatgt
     7681 taatttggta cagacctgtg gctgagtttg ctcaaagtat tcagagcaga attgtggagt
     7741 ggaaagagag attggacaaa gagtttagtt tgtcagtgta tcaaaaaatg aagtttaatg
     7801 tggctatggg aattggagtt ttagattggc taagaaacag tgatgatgat gatgaagaca
     7861 gccaggaaaa tgctgataaa aatgaagatg gtggggagaa gaacatggaa gactcagggc
     7921 atgaaacagg cattgattca cagtcccaag gctcatttca ggcccctcag tcctcacagt
     7981 ctgttcatga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8041 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacggg
     8101 gatcggatca atcactagag tcgcggccgc tttctcgagg ccatcgatgt ttaaacgatc
     8161 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     8221 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     8281 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     8341 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     8401 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     8461 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     8521 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     8581 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     8641 tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     8701 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaccgc gatcgcgaat
     8761 tcgcgatcgc catggctgtc agcgacgcgc tgctcccatc tttctccacg ttcgcgtctg
     8821 gcccggcggg aagggagaag acactgcgtc aagcaggtgc cccgaataac cgctggcggg
     8881 aggagctctc ccacatgaag cgacttcccc cagtgcttcc cggccgcccc tatgacctgg
     8941 cggcggcgac cgtggccaca gacctggaga gcggcggagc cggtgcggct tgcggcggta
     9001 gcaacctggc gcccctacct cggagagaga ccgaggagtt caacgatctc ctggacctgg
     9061 actttattct ctccaattcg ctgacccatc ctccggagtc agtggccgcc accgtgtcct
     9121 cgtcagcgtc agcctcctct tcgtcgtcgc cgtcgagcag cggccctgcc agcgcgccct
     9181 ccacctgcag cttcacctat ccgatccggg ccgggaacga cccgggcgtg gcgccgggcg
     9241 gcacgggcgg aggcctcctc tatggcaggg agtccgctcc ccctccgacg gctcccttca
     9301 acctggcgga catcaacgac gtgagcccct cgggcggctt catggccgag ctcctgcggc
     9361 cagaattgga cccggtgtac attccgccgc agcagccgca gccgccaggt ggcgggctga
     9421 tgggcaagtt cgtgctgaag gcgtcgctga gcgcccctgg cagcgagtac ggcagcccgt
     9481 cggtcatcag cgtcagcaaa ggcagccctg acggcagcca cccggtggtg gtggcgccct
     9541 acaacggcgg gccgccgcgc acgtgcccca agatcaagca ggaggcggtc tcttcgtgca
     9601 cccacttggg cgctggaccc cctctcagca atggccaccg gccggctgca cacgacttcc
     9661 ccctggggcg gcagctcccc agcaggacta ccccgaccct gggtcttgag gaagtgctga
     9721 gcagcaggga ctgtcaccct gccctgccgc ttcctcccgg cttccatccc cacccggggc
     9781 ccaattaccc atccttcctg cccgatcaga tgcagccgca agtcccgccg ctccattacc
     9841 aagagctcat gccacccggt tcctgcatgc cagaggagcc caagccaaag aggggaagac
     9901 gatcgtggcc ccggaaaagg accgccaccc acacttgtga ttacgcgggc tgcggcaaaa
     9961 cctacacaaa gagttcccat ctcaaggcac acctgcgaac ccacacaggt gagaaacctt
    10021 accactgtga ctgggacggc tgtggatgga aattcgcccg ctcagatgaa ctgaccaggc
    10081 actaccgtaa acacacgggg caccgcccgt tccagtgcca aaaatgcgac cgagcatttt
    10141 ccaggtcgga ccacctcgcc ttacacatga agaggcattt ttaaatccca gacagtggat
    10201 atgacccgtt tctagttcga aggatccaga catgataaga tacattgatg agtttggaca
    10261 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
    10321 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
    10381 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
    10441 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    10501 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10561 agacaagccc gtcaggcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg
    10621 atcgactcta gctagaggat cgatgccccg ccccggacga actaaacctg actacgacat
    10681 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
    10741 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
    10801 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
    10861 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
    10921 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
    10981 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
    11041 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
    11101 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
    11161 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
    11221 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
    11281 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
    11341 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
    11401 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
    11461 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
    11521 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
    11581 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
    11641 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
    11701 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
    11761 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
    11821 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
    11881 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
    11941 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
    12001 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
    12061 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
    12121 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
    12181 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
    12241 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
    12301 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
    12361 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
    12421 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
    12481 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
    12541 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
    12601 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
    12661 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    12721 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc
    12781 agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc
    12841 aagggggcgt gaattttcgc tgcttgtcct tttcctgctg gttgctccca ttcttaggtg
    12901 aatttaagga ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc
    12961 taatgttttc caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt
    13021 atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata
    13081 caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg
    13141 cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc
    13201 tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct
    13261 tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc
    13321 accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc
    13381 aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc
    13441 cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat
    13501 ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag
    13561 gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt
    13621 tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg
    13681 ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca
    13741 tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc
    13801 tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca
    13861 aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc
    13921 ggtggagacc cggatgatga tgactgacta ctgggactcc tgggcctctt ttctccacgt
    13981 ccacgacctc tccccctggc tctttcacga cttccccccc tggctctttc acgtcctcta
    14041 ccccggcggc ctccactacc tcctcgaccc cggcctccac tacctcctcg accccggcct
    14101 ccactgcctc ctcgaccccg gcctccacct cctgctcctg cccctcctgc tcctgcccct
    14161 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
    14221 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
    14281 gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
    14341 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgctcct
    14401 gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
    14461 gctcctgccc ctcctgcccc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc
    14521 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct
    14581 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
    14641 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
    14701 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
    14761 gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgctcc tgcccctccc
    14821 gctcctgctc ctgctcctgt tccaccgtgg gtccctttgc agccaatgca acttggacgt
    14881 ttttggggtc tccggacacc atctctatgt cttggccctg atcctgagcc gcccggggct
    14941 cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc
    15001 cctcttcttt gaggtccact gccgccggag ccttctggtc cagatgtgtc tcccttctct
    15061 cctaggccat ttccaggtcc tgtacctggc ccctcgtcag acatgattca cactaaaaga
    15121 gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc
    15181 ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc
    15241 tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga
    15301 aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc
    15361 ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc
    15421 ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg
    15481 tgaggatcag cttatcgatg ataagctgtc aaacatgaga attaattctt gaagacgaaa
    15541 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
    15601 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    15661 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    15721 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    15781 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    15841 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    15901 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    15961 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
    16021 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    16081 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    16141 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    16201 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    16261 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    16321 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    16381 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    16441 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    16501 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    16561 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    16621 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    16681 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    16741 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    16801 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    16861 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    16921 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    16981 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    17041 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    17101 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    17161 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    17221 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    17281 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    17341 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    17401 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
    17461 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
    17521 cg
//